Glycolipids: Function and Structure

Glycolipids: Function and Structure Introduction Marine fungi are saprophytic or heterotrophic form of filamentous spore forming eukaryote microorganisms are extensively lives in the marine or estuarine ecosystem. The characterization and diversity of the marine fungi can be studied by the direct observation of morphological structure and next generation sequencing. Taxonomically characterized marine fungi are belongs to either facultative or obligate forms. Facultative forms are originally sourced from terrestrial or fresh water region but they are able to colonize and adopt with the marine habitat and the obligate are extensively live in marine ecosystem (Kohlmeyer and Kohlmeyer, 1979). The fungi are extensively reported for the several biotechnological applications industrial utilization in enzymes, natural products and agriculture biocontrol etc. The marine fungus are taxonomically distinct (Jones et al., 2009), saline tolerant (Jennings, 1986), special biochemical properties (Damare et al., 2006). Interestingly, the marine fungi have the novelty than the obligate fungi and attracts in applications of omics (Damare et al., 2012). Generally, marine fungi can be isolated from the nutrient rich substrata such as decaying wood (harbour), coral reef (Le Campion- Alsumard et al., 1995), seagrases (Thirunavkkarasu, 2011) and mangrove ecosystem (Saravanakumar et al., 2012) and deep sea soil (Damare, 2007) are enhance distinct diversity of the obligate fungi (Sridhar, 2005). Among the marine substrate, mangrove is an second largest source for the isolation of obligate marine fungi (Ragukumar 2004). However, the biotechnological application of marine fungi differs with the terrestrial fungi due to their environmental adaptations and distributions. Many research focus on biotechnological utilization of natural products, enzymes, biocont rol, bioremediation, fuel conservation, waste management by using the marine fungi. A lot of structurally and pharmaceutically novel metabolites, isolated from marine fungi. However, this article focuses the glycollipids from the marine fungi and their properties, biological functions and applications. Glycolipids are a structurally very heterogenous group of membrane bound compound present in all living prokaryotic and eukaryotic organisams to human cells. The term of â€Å"glycolipid† is a compound contains one or more monosaccharides glycodidically linked in to a lipid (Brandenburg and Holst, 2005). Glycolipids are an essential constituent of cellular membrane and have the remarkable biological functions of cell aggregation or dissociation act as receptor of accepter to provide the contact. Several glycolipids has important role in immune system. Fungal glycolipidomics The glycolipids are interesting group of the compound occurred in cell wall of animals, microbes and plant sources (Pinto et al., 2008). The fungal glycolipids are composed of a sugar units usually glucose and galatose, hydrophobic ceramides, C19 sphingoid, C-9 metyl braches and unsaturated linkages with hydroxyhexadecanoic acids (Pinto et al., 2008). Glycoconjugates in fugal cell wall Glycoconjugates are composed of glycoprotins, peptides, glucons, polysaccharides, phosphoric acid, phospholipids, nitrogen and glycolipid molecules and found in the cell wall. Among the defining characteristics of fungal is cell wall complex architecture. Fungal cell walls are substantially thicker than bacterial cell walls and normally make up 10-30% of the biomass. They are freely permissible to small molecules and solute transport system and signalling receptors remains in cell membrane. A different cell wall found in the fungi comparing to animals and the role of these walls includes osmotic support, selective permeability and interaction with environment (Conzalexz et al., 2009). Fungal walls consist of covalently cross linked polysaccharides of ÃŽ ²-glycans and Chitin and several polysaccharides are covalently cross linked through glycosidic bonds (Pinto et al., 2008). Fungal glycolipids exterior Generally, the glycolipid molecules are found in cell membrane of all eukaryotic cell membranes, are contain the sugar called as glycolipids besides biologically produced biosurfactants are called as glycolipids. However, all type of glycolipids are biosurfactants but not all the biosurfactants are glycolipids (Mukherjee et al., 2006; Khopade et al., 2012). Simplest glycolipids contain the one or more sugars (Fig.) and complex glycolipids such as gangliosides contain a branch chain with several sugars. Cell membranes of the fungi have the many types membrane and are assembled from four compounds such as (i) phospholipids molecules, (ii) transmembrane proteins, (iii) inerter protein network, and (iv) cell surface markers are not identical. The glycolipids are formed in the cell wall of fungi by glycosylation in endoplasimic reticulam (ER) membrane sections and transfer the Golgi complex followed by plasma membrane (Fig). These add the sugar molecules chain to lipids called the sugar c oating lipids that extents the outside of fungal cells and differences were identified in glycolipids among fungal species and used as cell surface layer or marker besides glycolipids are also compound of the fatty acids contain carbohydrates, and nitrogen not phosphoric acids includes the certain compounds of the gangliosides, sulfolipids and salfatids (Pinto et al., 2008). The glycolipids are a marker for the cell identification of cell surface changes and are serving as fundamental building blocks of fungi, energy molecule or store, component of membrane constituents, signal molecule to interact the environmental compounds in through outer matrix, lectins, growth factor, and a potential factor of pathogenesis and immune responses (Hakomori, 1990; Springer and Lasky, 1991; Pinto et al., 2008). Moreover, the detail mechanism of role and properties of the glycolipids in fungus remain unclear. Marine fungal glycolipids Research on glycolipids from the marine resources has expanded the due attention due to its potential novelty in biotechnological applications. Muralidhar et al., (2003) have been reviewed the glycolipids from the marine resources such as algae (Lo et al., 2001), microorganisms: bacteria (Batrakov et al., 1998), fungi (Abraham et al., 1994), yeasts (Zinjarde and pant, 2002), actionbacteria (Kokare et al., 2007), sponges (pettit et al., 1999), gorgonians (Shin and Seo, 1995), sea anemones (Sugita et al., 1994), bryozoans (Ojika et al., 1997), tunicates (Loukaci et al., 2000), marine annelid (Noda et al., 1992), star fish (Sugiyama et al., 1988), sea cucumber (Higuchi et al., 1994), sea urchin (Babu et al., 1997) crinoids (Arao et al., 1999), molluscs (Yamaguchi et al., 1992), and marine crab (Asai et al., 2000). In terrestrial Fungus, in general yeasts have glycolipids as major constituents and are not the major compound in more fungal species. However, a high Glycolipids content of 11-16% of total lipids in Blastocladiella emersonii, the major compound of glycollipid is GalDAG and Gal2DAG (Mills and Cantino, 1974). The 61- 48 % of glycolipids is found in mycelia of Macrophomina phaseoline and the lower in the sclerotia (14-62%). However the glycolipids concentrations varied according the constituents of fermentation medium. The major compound of the fungal glycolipids identified as GalDAG and Gal2DAG based structural characterization. Further the major glycolipids of fungi is glycosphingolipids and D- glucosylceramides (Weete, 1980). Galactocerebrosides has been found in fungal species, of Aspergillus miger, C.utilis and S. cerevisae (Wagner and Zofcsik, 1969). Besides the fungal species Fusarium lini, Phycomycetes blakesleeanus and mushrooms are known to produce the glycolipids (Weiss et a l., 1973). Subsequently, the glycolipids are widely studied from Torulaspora delbruecki , Saccharomyes cerevisae, Candida glabrata, Kluyveromyes yarrowii, F. pedrosoi and K. polyporus (Saito et al., 2006 ; Pinto et al., 2008). The long chain sphingadinene has been first reported from Aspergillus oryzae (Fujino and Ohishi, 1976) and subsequently from Schizophyllum commune (Ballio et al., 1979), Fusicoccum amygdale (Ballio et al., 1979)), Clitocybe geotrope and Aspergillus fumigatus (Villas Boss et al., 1994), C. nebularis (Fodegel et al., 1986), A. niger(levery et al., 2000), A. versicolor (Walenkamp et al., 1999), Candida albicans (Matsubara et al., 1987), Acremonium chrysogenum (Sakaki et al., 2001), Cryptococcus neoformans (Rodrigues et al., 2000), Colletotrichum gloeosporioides ( de Silva et al., 2004), Fonsecaea pedrosoi (Nimrichter et al., 2005), Hansenula anomala (Ng et al., 1977), Fusarium sp. (Duarte et al., 1998), Histoplasma capulatum (Toledo et al., 2001), Kluyeromyces wa ltii (Takakuwa et al., 2002), paracoccidioides brasiliensis (Takahahi et al., 1996), Magnaporthe grisea (Koga et al., 2006), Pichia pastoris (Sakaki et al., 2001), Saccharomyces klyuyveri (Takakuwa et al., 2002), Pseudallescheria boydii (Pinto et al., 2002), Termitomyces albuminosus (Qi et al., 2002) Sporothrix schenkii (Toledo et al., 2001). In marine fungi, very few studies are available on glycolipids of marine fungi (Table.1); the marine white rot marine fungi Nia vibrissae is producer of glycolipids with inhibitory activity, the binding of endotoxin Lipopolysaccharide (LPS) to human endotoxin receptor (Helmholz et al., 1999). Marine fungi Gliocladium roseum KF-1040 is a producer of Roselipins can inhibit the enzyme diacylglycerol acyl transferase (Omura et al., 1999; Tomada et al., 1999; Tabata et al., 1999). Glycolipids derived from marine yeasts Calyptogena soyoae, Yarrowia lipolytica are effective on degradation of hydrocarbon (Zinjarde and pant, 2002; Konishi et al., 2010). Glycolipids synthesised form filamentous endosymbiotic Aspergillus ustus has the significant antimicrobial activity (Kiran et al., 2009). Several marine fungus such as Penicillum sp. F23-2 (Sun et al., 2009), Linincola laevis (Abraham et al., 1994), Fusarium sp (Li et al., 2002) and Microsphaeropsis olivacea (Keugen et al., 1996) are significa ntly produced the glycolipids with unknown application.

I’m Nobody Essay

â€Å"I’m Nobody! Who are You? † is a poem written by Emily Dickinson. The poem conveys the main idea of being alone, isolated from the society – or being â€Å"nobody†. This is partly influenced by the social gender status of Dickinson’s time – 19th century featured the inequality of sexes, where females were expected to stay at home and serve their husbands, thus disconnected from the society. As a result Dickinson had adapted and perhaps taken pleasure into being an outsider, whilst she found it boring to be part of the society – or to be a â€Å"somebody†. These are all various ideas reflected through Emily Dickinson’s poem. The poem has 2 stanzas – very typical of Emily Dickinson’s style. Her choice of language in this poem is also very simple and succinct – but at the same time meaningful and pithy. The first line, â€Å"I’m nobody! †, shows how Dickinson admits to be a â€Å"nobody† willingly. Being a â€Å"nobody† can mean an outsider – a person who is isolated, alienated from the rest of the world and society. The second phrase of the line – â€Å"Who are you? † shows that the poem is directly written to a target. This person – shown on the second line of the first stanza, is a â€Å"nobody† too – perhaps even a friend of Dickinson. The poet also realizes the fact that being a â€Å"nobody† is to be loathed by the society. This is shown in line 3, when she advises the other â€Å"nobody† not to tell, as she states in line 4 â€Å"They’d banish us†. The word â€Å"They† in the beginning of lines 3 and 4 suggests the rest of the society – people who are â€Å"somebody†, as opposed to Dickinson’s â€Å"nobody’. The use of dash in line 3 shows the furtiveness of the phrase â€Å"don’t tell! †, emphasizing the hatred or dislike they face from the society. The same effect is achieved by the use of exclamation mark in line 3. Lines 3 and 4 can also be interpreted as that after she finds another â€Å"nobody†, they are a pair – not longer belong to a group of â€Å"nobodies†. She does not want to be banished from the status of being â€Å"nobodies†. A conclusion can be drawn that she feels more secure to be a â€Å"nobody†; she feels comfortable to be treated as a â€Å"nobody† by the rest of the society. The second stanza sees a noticeable change in Dickinson’s tone. The repetitions of â€Å"How† and â€Å"To† in the beginning of each line give a more secure and commanding tone. This can be explained by the â€Å"discovery† of another â€Å"nobody† stated by the poet in the first stanza – she feels more assured that to be a â€Å"nobody† is not too unacceptable as she is not the only one. Also perhaps because she feels more secure to be with a person who feels the same as she does and understands her, she is more willing and daring to express her more inner feelings. The poet states that it is â€Å"dreary to be somebody† in line 5 of the second stanza. â€Å"Dreary† refers to being boring or dull; and â€Å"to be somebody† suggests to be recognized by the society and belong to it. Hence Dickinson is suggesting that to be part of a society is tedious and meaningless to her, which can also implies being a â€Å"nobody† is the contrary – interesting and meaningful. A further implication may be that being â€Å"nobody† allows her to write poetry – as it is not part of the â€Å"traditional role† of women at her time to write poetry, and instead women were expected to serve only their husbands. However, being a â€Å"nobody† in the society helps her to escape her role of being a â€Å"women†, granting her freedom instead. Hence we can see that poetry is meaningful to her. She also compares that to be â€Å"somebody† is like to be â€Å"public†, another indication that Dickinson likes isolation – which is proven correctly as one would learn that she locked herself in her house for the majority of her life. Dickinson uses a simile to compare â€Å"somebody† to a â€Å"frog† in line 6. This can be explained by the fact that â€Å"frogs† are considered noisy with the sound they generate. Combining with line 8, â€Å"To an admiring bog! †, it suggests the idea that frogs create noise to be noticed – but only by â€Å"an admiring bog†. A bog is the environment in which a frog dwells – this creates an irony. Emily Dickinson is suggesting that although being a â€Å"somebody† means being noticed by the public, but the public to â€Å"somebody† is like a bog to a frog – it is not really a relationship, or friendship, as no one would say that a bog is the friend of a frog. Also the word â€Å"admiring† creates a whole sense of sarcasm to the idea – and the technique of personification is used to describe the â€Å"bog† as well, perhaps to emphasize the sarcasm of the simile. Overall the poet suggests that to be â€Å"somebody† might mean to be well-known, accepted by the society; however the relationships are often shallow, distanced or impersonal. The rhyming of the words â€Å"frog† and â€Å"bog† also suggests a congenial relationship between â€Å"Somebody† and her targeted audience – conveying the idea that the poem is a direct criticism against the â€Å"somebody† – the general public. Through the use of contrast and irony between â€Å"Nobody† and â€Å"Somebody†, her strong will to be a â€Å"nobody† is shown, as well as her despise towards â€Å"somebody†. This poem reflects Emily Dickinson’s life and perhaps her more inner and cryptic feelings – it was probably written from the heart. She imprisoned herself for the most of her life, completely isolated from the rest of the world. This may contribute to the reason why she thinks being an â€Å"outsider† is better than being â€Å"somebody†, and that she does not value â€Å"friendship† in the same way as normal people do. However at the same time it was also proven in the poem – for instance she found another â€Å"nobody† in the first stanza – that she is not totally a recluse, and that she treasures the very few friendships she had.

What Everyone Else Does When It Comes to Best Essays and What You Should Do Different

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Informative Speech - 961 Words

Informative Outline Topic: â€Å"Postpartum Depression† General Purpose: To inform Specific Purpose: To inform the audience about Postpartum Depression Thesis: Postpartum depression is a mood disorder that can greatly effect new mothers. Knowing how to recognize their symptoms and treating it can greatly increase chances of a healthy, happy living. Attention Getter: Imagine a bright sunny morning, lite clouds low chances of rain which is a great example of a perfect day. You receive a call from someone who is about to have a baby. Who can believe an already perfect day can get any better. The mother is in labor and the baby is due any second now. The most joyous, exciting, life-changing and memorable moment in anyone’s life is about to†¦show more content†¦In the 2006 book Postpartum Mood and Anxiety Disorders: A Clinician’s Guide authored by Beck and Driscoll, states certain risk factors such as socioeconomic status, ethnicity, education level and self-esteem can also play a role in the development of this depression. B. There are several mood disorders that falls under the umbrella of PPD which makes it vital to decipher between them. 1. Baby Blues, the most common and mildest form of mood disorder can last up to two weeks after having a baby without the mother needing treatment. a. Its very name describes what it entails: crying, anxiety, and sadness. b. Davidson stated in her 2012 book A Nurse’s Guide to Women’s Mental Health that â€Å"It is estimated that 50-80 percent of women suffers from some form of baby blues after birth†. (pg.175) 2. Postpartum Depression is more intense and last longer than Baby Blues. It takes effect a few weeks to a year after a child is born. 3. Postpartum Psychosis is a rare but the most severe form of postpartum depression. a. It can begin at any time during the first year after having a newborn and due to its severity and length it must be diagnosed and treated as soon as possible. b. This form involves a mother having hallucinations and delusions. Transition (Now that I have told you what postpartum depression is letShow MoreRelatedInformative Speech Essay855 Words   |  4 Pagestype of speech would the speaker choose. Well chapter 13 contents the creative process for informative speaking. What informative speaking is how to choose a focused informative topic, how to conduct a research and informative outline? The chapter also contains how to organize the body, introduction, and conclusion of the informative speech. Lastly chapter 13 contents explain how to prepare to present the speech and evaluate and informative speech. 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Thesis statement: Gun control will create laws and licensing to possess a gun to help decrease crime and fatalities, but also people believe gun control is going against our constitutionalRead Moreinformative speech1255 Words   |  6 Pagesï » ¿ Title: Information Speech Outline â€Å"The Evolution of Animation† Subject Code: USEL 23 - Speech Communication Prepared For: Datin Minda Hassan Prepared By: Nur Rabiatul Adawiyah Bt Mohd Nor Kamarudin (1411170007) Class: Oregon Program: ADFP Summer 2014 Preparation Outline Informative Speech â€Å"The Evolution of Animation – From 2D Animation to 3D Animation† General Purpose: To inform Specific Purpose: To inform my audience the evolution of animation. Central

Obesity Is A Complex Process - 953 Words

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Beside this health campaign, a consistent nutritional label system was launched in 2013 by the Public Health Minister Anna Soubry. This system combines colour coding (red, amber, green) and nutritional information to demonstrate the amount of sugar, salt, fat and calorie contain in the food products. The colour-coding will help people to comprehend the level of nutrients in the food that they are eating (Gov.uk. 2013). Another tackling obesity strategy has been published in October 2011 â€Å"Healthy Lives, Healthy People: A call to action on obesity in England†, with a new target in reducing the excess weight of theShow MoreRelatedObesity Essay1253 Words   |  6 Pagesand adolescents with obesity and obesity related diseases reported to have systemic inflammation and evidence of endothelial activation. 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It delves into the components that contribute to this menacing epidemic that only continues to get worse. The documentary builds on the stories of four young American children from all over the country that are severely obese. One of the kids, at 14 years of age, weighs over 400 pounds. Fed Up tries to answer one simple question with a complex and scary

Services Marketing and Customer Relationship Free Essays

Spring 2010 (Jan-Jun) Master of Business Administration-MBA Semester 4 MK0006 – Services Marketing and Customer Relationship Management – 2 Credits (Book ID: xxxxxxx) Assignment Set- 1 (30 Marks) Note: Each question carries 10 Marks. Answer all the questions. Q. We will write a custom essay sample on Services Marketing and Customer Relationship or any similar topic only for you Order Now 1 a. What do you mean by physical evidence in services? Give examples. (5 marks) b. Write a note on service development. (5 marks) Q. 2 a. Briefly explain the terms â€Å"customer expectation† and â€Å"customer perception†. (6 marks) b. What are the different bases for segmentation? 4 marks) Q. 3 Recall the different services offered in a bank. Evaluate the different characteristics of services that you found excellent, average and poor in the particular bank. How do you think the bank can improve its services? (10 marks) Spring 2010 (Jan-Jun) Master of Business Administration-MBA Semester 4 MK0006 – Services Marketing and Customer Relationship Management – 2 Credits (Book ID: xxxxxxx) Assignment Set- 2 (30 Marks) Note: Each question carries 10 Marks. Answer all the questions. Q. 1 a. Discuss strategy formulation and implementation in services. (4 marks) b. Explain the scope of CRM. (6 marks) Q. 2 a. What do you mean by CRM? What are the emerging trends in CRM? (5 marks) b. Write a note on service quality with an illustration. (5 marks) Q. 3 Determine a service marketing mix for an established airline. Assume that the airline operates in more than 30 different countries and its customers are mostly regular ones. How can it distinguish its services from other competing airlines, so as to attract newer passengers? (10 marks) How to cite Services Marketing and Customer Relationship, Essays

Representations Of scape In Art Essay Example For Students

Representations Of scape In Art Essay It seems that nowadays artists are no longer limiting themselves to representing their surroundings by simply sketching or photographing them, exactly as they are. Contemporary art is now promoting a new concept, that encourages the artist to interpret their surroundings and depict them in their own unique way - a concept known as, Scape. Scape is rapidly becoming more popular than ever in contemporary art, which I believe is due to its diversity and the ability it gives artists to escape into their own worlds; real or not - and represent it, in their own unique way. With scape, the artist has no limitations and is offered more freedom for self expression - artworks can be based upon imagination, dream or reality and are often greatly influenced by the artists own beliefs and perceptions. The different ways in which scape is represented therefore are infinite. The only real boundary on the way in which scape can represented is the artists imagination and own choice - while some artists may choose to portray their surroundings in a more abstract way, others may simply choose to sketch, for example, a mountain. Scape also enables the viewer the opportunity to better interpret what the artist feels about their surroundings à - i.e. if the artist perceives something in a negative way, we can almost surely conclude that the way in which they portray that something, will be in a negative manner (using dark colours, distorting etc.) This form of art is very easily manipulated, so as to represent what the artist wants according to his/her perceptions, beliefs, ideologies etc. An example of this can be seen in Brett Whiteleys, Van Gough inspired, Night Cafe © (1972). Whiteley studied Van Gough for a number of years and he says that during this period of his life as an artist his perceptions of his surroundings changed significantly, which lead him to create the Van Gough inspired series. Brett Whiteley- Night Cafe © (1972) Oil on board Art Gallery of NSW He took a scene, with a pool table and cafe © like setting, altered the lines to create the illusion that they were all heading to a single vanishing point and gave all the light sources in the scene (street lamps etc) a circular glow. Giving an obvious Van Gough resemblance and an overall effect of a very fast moving, vibrant and dynamic image (the colour choice adds to this also). As you can see from this example of a Contemporary scape artist, the surroundings that Whiteley chose to portray, were manipulate to represent his own perceptions and love of Van Goughs work, to create a scape piece that is therefore very unique and personal. Proving just how diverse scape is, and the numerous ways in which it can be represented; depending on the artists own choice. This work was not influenced by personal beliefs or experiences as such, but still gives a good example of how scape work can be represented in different ways, influenced by what the artists likes and dislikes are - i.e. Whiteley Liked Van Goughs work, and therefore his surroundings were influenced by that. Another example of scape, which has much more influence from personal ideologies, is Christo and Jean-Claudes Wrapped Trees (1997). There artwork is largely based on temporality; with piece usually only lasting around 15 days. In Wrapped Trees, specialised fabric covers were measured and made for hundreds of trees and were then draped and fastened over them. Adding, what the husband-wife team said, a sense of vulnerability, fragility and urgency to their artwork, as it could only last for a limited time. .udaed9693b458e4f8c064bf4b735ce8b0 , .udaed9693b458e4f8c064bf4b735ce8b0 .postImageUrl , .udaed9693b458e4f8c064bf4b735ce8b0 .centered-text-area { min-height: 80px; position: relative; } .udaed9693b458e4f8c064bf4b735ce8b0 , .udaed9693b458e4f8c064bf4b735ce8b0:hover , .udaed9693b458e4f8c064bf4b735ce8b0:visited , .udaed9693b458e4f8c064bf4b735ce8b0:active { border:0!important; } .udaed9693b458e4f8c064bf4b735ce8b0 .clearfix:after { content: ""; display: table; clear: both; } .udaed9693b458e4f8c064bf4b735ce8b0 { display: block; transition: background-color 250ms; webkit-transition: background-color 250ms; width: 100%; opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #95A5A6; } .udaed9693b458e4f8c064bf4b735ce8b0:active , .udaed9693b458e4f8c064bf4b735ce8b0:hover { opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #2C3E50; } .udaed9693b458e4f8c064bf4b735ce8b0 .centered-text-area { width: 100%; position: relative ; } .udaed9693b458e4f8c064bf4b735ce8b0 .ctaText { border-bottom: 0 solid #fff; color: #2980B9; font-size: 16px; font-weight: bold; margin: 0; padding: 0; text-decoration: underline; } .udaed9693b458e4f8c064bf4b735ce8b0 .postTitle { color: #FFFFFF; font-size: 16px; font-weight: 600; margin: 0; padding: 0; width: 100%; } .udaed9693b458e4f8c064bf4b735ce8b0 .ctaButton { background-color: #7F8C8D!important; color: #2980B9; border: none; border-radius: 3px; box-shadow: none; font-size: 14px; font-weight: bold; line-height: 26px; moz-border-radius: 3px; text-align: center; text-decoration: none; text-shadow: none; width: 80px; min-height: 80px; background: url(https://artscolumbia.org/wp-content/plugins/intelly-related-posts/assets/images/simple-arrow.png)no-repeat; position: absolute; right: 0; top: 0; } .udaed9693b458e4f8c064bf4b735ce8b0:hover .ctaButton { background-color: #34495E!important; } .udaed9693b458e4f8c064bf4b735ce8b0 .centered-text { display: table; height: 80px; padding-left : 18px; top: 0; } .udaed9693b458e4f8c064bf4b735ce8b0 .udaed9693b458e4f8c064bf4b735ce8b0-content { display: table-cell; margin: 0; padding: 0; padding-right: 108px; position: relative; vertical-align: middle; width: 100%; } .udaed9693b458e4f8c064bf4b735ce8b0:after { content: ""; display: block; clear: both; } READ: FCC vs Pacifica Broadcasting Foundation EssayThe couple have a love of what will not last and the unknown; and are fascinated with the ways in which humans cherish and strive to protect things that are on a time limit (eg. Life, childhood), and they want their art to have that same effect on people whilst Creating a gentle disturbance (Christo) by creating there impermanent artworks in places no one would expect. As you can see, again, an artists personal ideologies tend to shape and influence the ways in which they choose to represent their art - and scape allows them to do this easily and effectively. Christo and Jean-Claude go for a more hands on approach to representing their views of scape, and is just one more of the numerous ways in which an artist may choose to do so. Christo and Jean-Claudes work makes me think about both art and the environment in a new and unexpected way.